(i) Method of working : He maintained the statistical records of all the experiments and analysed them. He selected genetically pure (pure breed line) and purity was tested by self-crossing the progeny for several generations.

(ii) Selection of material : Mendel selected garden pea as his experimental material because it has the following advantages.
It was an annual plant. Its short life�cycle made it possible to study several generations within a short period and has perfect bisexual flowers containing both male and female parts. The flowers are predominantly self-pollinating because of self-fertilization, plants are homozygous. It is, therefore, easy to get pure lines for several generations and also easy to cross because pollens from one plant can be introduced to the stigma of another plant by removing anthers (emasculation) and bagging. In addition to that there was one reason more for his success. He studied seven pairs of characters which were present on four different pairs of chromosomes.

(iii) Selection of traits : Mendel selected seven pairs of contrasting characters as listed in the table. Luckily all were related as dominant and recessive.

# (i) Monohybrid cross : Experiments with garden pea for single pair of contrasting characters.

(a) Procedure : Mendel crossed pure tall and dwarf plants. The plants belonged to F1 generation all tall were self-pollinated. The plants of F2 generation were both tall and dwarf, in approximate 3:1 ratio phenotypically and 1:2:1 genotypically. On, self-pollination, the tall plants of F2 only 1/3�rd breed true for tallness, the rest 2/3�rd produced tall and dwarf in the ratio of 3:1 (F3 generation). It means F2 generation consisted of three types of plants (instead of apparent two types) �
Tall homozygous (Pure)
1 25% TT
Tall heterozygous (Hybrid)
2 50% Tt
Dwarf homozygous (Pure)
1 25% tt
Hence it is to be said that in F2 generation 50% plants passes parental combination while 50% are new combination.

(b) Mendel�s explanation : Mendel explained above results by presuming that Tallness and dwarfness are determined by a pair of contrasting factors or determiners (now these are called genes). A plant is tall because it possesses determiners for tallness (represented by T) and a plant is dwarf because it has determiners for dwarfness (represented by t). These determiners occur in pairs and are received one from either parent. On the basis of this behaviour the tallness is described as dominant character and dwarfness as recessive (law of dominance). The determiners are never contaminated. When gametes are formed, these unit factors segregate so that each gamete gets only one of the two alternative factors. When F1 hybrids (Tt) are self pollinated the two entities separate out and unite independently producing tall and dwarf plants (law of segregation).

#(ii) Dihybrid cross (Crosses involving two pairs of contrasting characters)

(a) Procedure : Later on Mendel conducted experiments to study the segregation and transmission of two pairs of contrasting characters at a time. Mendel found that a cross between round yellow and wrinkled green seeds (P1) produced only round and yellow seeds in F1 generation, but in F2 four types of combinations were observed. These are
Round yellow 9 Parental combinations
Round green 3 Non-parental combinations
Wrinkled yellow 3 Non-parental combination
Wrinkled green 1 Parental combination
Thus the offsprings of F2 generation were produced in the ratio of [ 9 : 3 : 3 : 1 ] phenotypically and [ 1 : 2 : 2 : 4 : 1 : 2 : 1 : 2 : 1 ] genotypically. This ratio is called dihybrid ratio.
The results can be represented as follows:
Mendel represented round character of seed by R and wrinkled by r. Similarly he designated the yellow character by Y and green by y. Therefore, it was a cross between RRYY and rryy.

(b) Mendel�s explanation : Mendel explained the results by assuming that the round and yellow characters are dominant over wrinkled and green so that all the F�1 offsprings are round yellow. In F2-generation since all the four characters were assorted out independent of the others, he said that a pair of alternating or contrasting characters behave independently of the other pair i.e., seed colour is independent of seed coat.

Therefore, at the time of gamete formation genes for round or wrinkled character of seed coat assorted out independently of the yellow or green colour of the seed. As a result four types of gametes with two old and two new combinations i.e., RY, ry Ry, rY are formed from the F1 hybrid. These four types of gametes on random mating produced four types of offsprings in the ratio of [ 9:3:3:1 ] in F2 generation (Law of Independent Assortment).

# (iii) Trihybrid cross : The offsprings shows [ 27 : 9 : 9 : 9 : 3 : 3 : 3 : 1 ] ratio is found in trihybrid cross. This suggests that a di, tri, or polyhybrid cross is actually a combination of respectively two, three or more monohybrid crosses operating together.

(iv) MENDAL'S LAW OF INHERITANCE : Mendel's law are still true because these take place in sexually reproducing organisms or parents are of pure breeding. He enunciated two major laws of inheritance i.e., law of segregation and law of independent assortment.

(a) LAW OF SEGREGATION (Purity of gametes) : The law of segregation states that when a pair of contrasting factors or genes or allelomorphs are brought together in a heterozygote (hybrid) the two members of the allelic pair remain together without being contaminated and when gametes are formed from the hybrid, the two separate out from each other and only one enters each gamete as seen in monohybrid and dihybrid cross. That is why the law of segregation is also described as law of purity of gametes.

(b) LAW OF INDEPENDENT ASSORTMENT : If the inheritance of more than one pair of characters (two pairs or more) is studied simultaneously, the factors or genes for each pair of characters assort out independently of the other pairs. Mendel formulated this law from the results of a dihybrid cross.