Biology MOLECULAR BASIS OF INHERITANCE
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MOLECULAR BASIS OF INHERITANCE

Genes expression and its regulation

(1) Gene expression in prokaryotes : Gene expression refers to the molecular mechainism by which a gene expresses a phenotype by synthesizing a protein or an enzyme. Which determines the character. The gene contains the blue print or the information for the protein or an enzyme.
The category includes mechanism involved in the rapid turn-on and turn-off gene expression in response to environmental changes. Regulatory mechanism of this type are very important in microorganisms, because of the frequent exposure of these organisms to sudden changes in environment.
Gene concept can be studied by operon model. Operon are segment of genetic material which function as regulated unit that can be switched on and switched off, which was given by French scientists. Jacob and Monod (1961) working at Pasteur institute. They were studying lactose utilisation in mutants of E.coli. An operon consists of one to several structural genes (three in lac operon and five in tryptophan operon of Escherichia coli, nine in histidine operon of Salmonella typhimurium), an operator gene a promoter gene a regulator gene, a repressor and inducer or corepressor. Operons are of two types, inducible and repressible.

(i) Inducible operon system / lac operon system : An inducible operon system is that regulated genetic material which remains switched off normally but becomes operational in the presence of an inducer. It occurs in catabolic pathways. The components are :–
# (a) Structural genes : They are genes, which produce mRNAs for forming polypeptides/proteins/enzymes. Lac operon of Escherichia coli has three structural genes-Z (produces enzyme -galactosidase for splitting lactose/galactoside in to glucose and galactose) Y (produces enzyme galactoside permease required in entry of lactose/galactoside) and A (produces enzyme galactoside acetylase/transacetylase without any function in E.coli). The three structural genes of lac operon produce a single polycistronic mRNA. The three enzymes are, however, produced in different concentration.

# (b) Operator gene : It gives passage to RNA polymerase when the structural genes are to express themselves. Normally, it is covered by a repressor. Operator gene of lac operon is small, made of 27 base pairs.

# (c) Promoter gene : It is recognition centre / initiation point for RNA polymerase of the operon.

# (d) Regulator gene (i Gene) : It produces a repressor that binds to operator gene for keeping it nonfunctional (preventing RNA polymerase to pass from promoter to structural genes).

# (e) Repressor : It is a small protein formed by regulator gene. Which binds to operator gene and blocks passage of RNA polymerase towards structural enzymes. Repressor has two allosteric sites, one for attaching to operator gene and second for binding to inducer. Repressor of lac operon has a molecular weight of 160,000 and 4 subunit of 40,000 each.

# (f) Inducer : It is a chemical which attaches to repressor, changes the shape of operator binding site so that repressor no more remain attached to operator.
Lactose/galctoside is inducer of lac operon. As soon as the operator gene becomes free, RNA polymerase is recognised by promoter gene. cAMP is required, RNA polymerase passes over the operator gene and then reaches the area of structural genes. Here it catalyses transcription of mRNAs.

(2) Gene expression in eukaryotes

Gene expression in eukaryotes : In regulation of gene expression in eukaryotes the chromosomal proteins play important role. The chromosomal proteins are of two types. They are histones and non-histones. The regulation of gene expression involves an interaction between histones and non-histones. Histones inhibit protein synthesis and non-histones induce RNA synthesis. There are four main steps in the expression of genes. Hence regulation is brought about by the regulation and modification of one or more of these steps. They are
(i) Replication
(ii) Transcription
(iii) Processing
(iv) Translation

# (i) Regulation of replication : Differential gene expression is achieved by gene amplification.
# (ii) Regulation of transcription : The regulation of the expression of gene is mainly done at transcription. Hybridization experiments clearly show that production of specialised protein is due to differential gene transcription.
# (iii) Regulation of the processing level : Some of the RNA synthesized in the nucleus are destroyed without leaving the nucleus. 80% of the nuclear RNA has no equivalent in the cytoplasm and only 20% if the nuclear RNA is identical in the cytoplasm. All the genes in a cell are transcribed into mRNA at all times, but the mRNA produced by some genes is destroyed rapidly. But the mRNA modeled on other genes are stabilized and only these mRNAs are passed into the cytoplasm.
# (iv) Regulation of translation : The control of mRNA-translation is a fundamental phenomenon. In sea-urchin eggs fertilisation is followed by a tremendous increase in protein synthesis; but in the unfertilised egg, there is no protein synthesis. Still the unfertilised egg has complete machinery (i.e., amino acids, ribosomes, mRNA) for protein synthesis. There are two model for regulation in eukaryotes.
(a) Frenster's model : According to 1965, The histones act as repressor's during protein synthesis.
(b) Britten Davidson model : This is also called gene battery model or operon-operator model. It was proposed by Britten and Davidson in 1969. They have been proposed four type of genes namely integrator. sensor, producer and receptor.
 
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