● It was `color{brown}"George Gamow"`, a physicist who argued that since there are only `color{violet}"4 bases"` and if they have to code for `color{violet}"20 amino acids"`, the code should constitute a `color{violet}"combination of bases"`.
● He suggested that in order to code for all the 20 amino acids, the code should be made up of `color{violet}"three nucleotides"`.
● This was a very bold proposition, because a `color{violet}"permutation combination"` of `4^3` (4 × 4 × 4) would generate `color{violet}"64 codons"`; generating many more codons than required.
● Providing proof that the `color{violet}"codon was a triplet"`, was a more daunting task.
● The `color{violet}"chemical method"` developed by `color{brown}"Har Gobind Khorana"` was instrumental in synthesising RNA molecules with `color{violet}"defined combinations"` of bases (homopolymers and copolymers).
● `color{brown}"Marshall Nirenberg’s"` `color{violet}"cell-free system"` for protein synthesis finally helped the code to be deciphered.
● Several `color{violet}"Ochoa enzyme"` (`color{violet}"polynucleotide phosphorylase"`) was also helpful in polymerising RNA with defined sequences in a template independent manner (enzymatic synthesis of RNA).
● Finally a `color{violet}"checker-board"` for genetic code was prepared
● It was `color{brown}"George Gamow"`, a physicist who argued that since there are only `color{violet}"4 bases"` and if they have to code for `color{violet}"20 amino acids"`, the code should constitute a `color{violet}"combination of bases"`.
● He suggested that in order to code for all the 20 amino acids, the code should be made up of `color{violet}"three nucleotides"`.
● This was a very bold proposition, because a `color{violet}"permutation combination"` of `4^3` (4 × 4 × 4) would generate `color{violet}"64 codons"`; generating many more codons than required.
● Providing proof that the `color{violet}"codon was a triplet"`, was a more daunting task.
● The `color{violet}"chemical method"` developed by `color{brown}"Har Gobind Khorana"` was instrumental in synthesising RNA molecules with `color{violet}"defined combinations"` of bases (homopolymers and copolymers).
● `color{brown}"Marshall Nirenberg’s"` `color{violet}"cell-free system"` for protein synthesis finally helped the code to be deciphered.
● Several `color{violet}"Ochoa enzyme"` (`color{violet}"polynucleotide phosphorylase"`) was also helpful in polymerising RNA with defined sequences in a template independent manner (enzymatic synthesis of RNA).
● Finally a `color{violet}"checker-board"` for genetic code was prepared