● When a `color{Violet}"piece of alien DNA"` is inserted into a `color{Violet}"cloning vector"` and transferred it into a bacterial, plant or animal cell, the `color{Violet}"alien DNA gets multiplied"`.
● In almost all `color{Violet}"recominant technologies"`, the ultimate aim is to produce a `color{Violet}"desirable protein"`.
● Hence, there is a need for the `color{Violet}"recombinant DNA"` to be `color{Violet}"expressed"`.
● The foreign gene gets expressed `color{Violet}"under appropriate conditions"`.
● The `color{Violet}"expression of foreign genes"` in host cells involve understanding many `color{Violet}"technical details"`.
● After having `color{Violet}"cloned the gene"` of interest and having `color{Violet}"optimised the conditions"` to induce the expression of the target protein, one has to consider `color{Violet}"producing it on a large scale"`.
● If any `color{Violet}"protein encoding gene"` is expressed in a `color{Violet}"heterologous host"`, is called a `color{Brown}"recombinant protein"`.
● The cells harbouring `color{Violet}"cloned genes of interest"` may be grown on a small scale in the `color{Violet}"laboratory"`.
● The cultures may be used for extracting the desired protein and then purifying it by using different separation techniques.
● The cells can also be multiplied in a `color{Brown}"continuous culture system"` wherein the `color{Violet}"used medium"` is drained out from one side while `color{Violet}"fresh medium"` is added from the other to maintain the cells in their physiologically most active `color{Violet}"log/exponential phase"`.
● This type of culturing method produces a `color{Violet}"larger biomass"` leading to higher yields of `color{Violet}"desired protein"`.
`color{Violet}"Small volume cultures"` cannot yield appreciable quantities of products.
● When a `color{Violet}"piece of alien DNA"` is inserted into a `color{Violet}"cloning vector"` and transferred it into a bacterial, plant or animal cell, the `color{Violet}"alien DNA gets multiplied"`.
● In almost all `color{Violet}"recominant technologies"`, the ultimate aim is to produce a `color{Violet}"desirable protein"`.
● Hence, there is a need for the `color{Violet}"recombinant DNA"` to be `color{Violet}"expressed"`.
● The foreign gene gets expressed `color{Violet}"under appropriate conditions"`.
● The `color{Violet}"expression of foreign genes"` in host cells involve understanding many `color{Violet}"technical details"`.
● After having `color{Violet}"cloned the gene"` of interest and having `color{Violet}"optimised the conditions"` to induce the expression of the target protein, one has to consider `color{Violet}"producing it on a large scale"`.
● If any `color{Violet}"protein encoding gene"` is expressed in a `color{Violet}"heterologous host"`, is called a `color{Brown}"recombinant protein"`.
● The cells harbouring `color{Violet}"cloned genes of interest"` may be grown on a small scale in the `color{Violet}"laboratory"`.
● The cultures may be used for extracting the desired protein and then purifying it by using different separation techniques.
● The cells can also be multiplied in a `color{Brown}"continuous culture system"` wherein the `color{Violet}"used medium"` is drained out from one side while `color{Violet}"fresh medium"` is added from the other to maintain the cells in their physiologically most active `color{Violet}"log/exponential phase"`.
● This type of culturing method produces a `color{Violet}"larger biomass"` leading to higher yields of `color{Violet}"desired protein"`.
`color{Violet}"Small volume cultures"` cannot yield appreciable quantities of products.